Original Article
Pak J Nucl Med. 2018; 8(1): 31-37

In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications

Authors: Hameed Ullah, Syed Qaiser Shah, Aakif Ullah Khan, Shakil Ahmad, Muhammad Rauf Khattak.

View PDF DOI: 10.24911/PJNMed.175-1522742093

Abstract

Background: A convenient method was developed for the preparation of pentavalent 99mTc dimercaptosuccinic acid using a commercially available meso-2,3-dimercaptosuccinic acid (DMSA) kit. The effects of various parameters, including incubation time, pH, and concentration of sodium bicarbonate on radiochemical purity (RCP) yield were evaluated. To determine tri- and pentavalent fractions, RCP yield of the labeled DMSA was measured using different solvent systems. Methods: The RCP yield was studied using a thin-layer chromatography on silica gel as the stationary phase and n-butanol/acetic acid/distilled water (3:2:2) as the mobile phase. Results: It was observed that hydrolyzed 99mTcO2 and 99mTc(III)-DMSA remained at the origin (Rf = 00.1), and 99mTc(V)-DMSA at (Rf = 0.50.6), while free pertechnetate, 99mTcO−4 moved to the solvent front (Rf = 0.91). However, in acetone, 99mTc(III)-DMSA, 99mTc(V)DMSA, and hydrolyzed 99mTcO2 stayed at the origin (Rf = 00.1), while free pertechnetate, 99mTcO−4 moved to the solvent front (Rf = 0.91). When developed with 5% glycine, 99mTcO2 stayed at the origin and the other species migrated to the solvent front. The labeling efficiency of the 99mTc(V)DMSA was 96.6 0.5% and was stable for 24 hours at room temperature. Conclusion: Higher labeling yield, easy preparation with low cost, and good stability make 99mTc(V)-DMSA, a promising radio drug for clinical use.

Keywords:   Medullary thyroid cancer, 99mTc(V)-DMSA, radiochemical purity, thin-layer chromatography.


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In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications


Authors
Hameed Ullah
Institute of Radiotherapy and Nuclear Medicine (IRNUM), Peshawar, Pakistan
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Syed Qaiser Shah
Institute of Chemical Sciences (ICS), University of Peshawar, KPK, Pakistan
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Aakif Ullah Khan
Swat Institute of Nuclear Medicine and Radiotherapy (SINOR), Swat, Pakistan
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Shakil Ahmad
Institute of Radiotherapy and Nuclear Medicine (IRNUM), Peshawar, Pakistan
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Muhammad Rauf Khattak
Institute of Radiotherapy and Nuclear Medicine (IRNUM), Peshawar, Pakistan
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Correspondence to:
Hameed ullah. Hameed ullah, Institute of Radiotherapy and Nuclear Medicine (IRNUM), Peshawar, Pakistan; hameedullahirnum@gmail.com

Publication history
Published online 01 Jan 1970
Received 04 Apr 2018
Revised 13 Jul 2018
Accepted 01 Nov 2018
Published in print 10 Dec 2019
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How to cite this article

Pubmed Style

Ullah H, Shah SQ, Khan AU, Ahmad S, Khattak MR. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pak J Nucl Med. 2018; 8(1): 31-37. doi:10.24911/PJNMed.175-1522742093


Web Style

Ullah H, Shah SQ, Khan AU, Ahmad S, Khattak MR. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. http://www.pjnmed.com/?mno=295574 [Access: April 08, 2020]. doi:10.24911/PJNMed.175-1522742093


AMA (American Medical Association) Style

Ullah H, Shah SQ, Khan AU, Ahmad S, Khattak MR. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pak J Nucl Med. 2018; 8(1): 31-37. doi:10.24911/PJNMed.175-1522742093


Vancouver/ICMJE Style

Ullah H, Shah SQ, Khan AU, Ahmad S, Khattak MR. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pak J Nucl Med. (2018), [cited April 08, 2020]; 8(1): 31-37. doi:10.24911/PJNMed.175-1522742093


Harvard Style

Ullah, H., Shah, . S. Q., Khan, . A. U., Ahmad, . S. & Khattak, . M. R. (2018) In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pak J Nucl Med, 8 (1), 31-37. doi:10.24911/PJNMed.175-1522742093


Turabian Style

Ullah, Hameed, Syed Qaiser Shah, Aakif Ullah Khan, Shakil Ahmad, and Muhammad Rauf Khattak. 2018. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pakistan Journal of Nuclear Medicine, 8 (1), 31-37. doi:10.24911/PJNMed.175-1522742093


Chicago Style

Ullah, Hameed, Syed Qaiser Shah, Aakif Ullah Khan, Shakil Ahmad, and Muhammad Rauf Khattak. "In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications." Pakistan Journal of Nuclear Medicine 8 (2018), 31-37. doi:10.24911/PJNMed.175-1522742093


MLA (The Modern Language Association) Style

Ullah, Hameed, Syed Qaiser Shah, Aakif Ullah Khan, Shakil Ahmad, and Muhammad Rauf Khattak. "In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications." Pakistan Journal of Nuclear Medicine 8.1 (2018), 31-37. Print. doi:10.24911/PJNMed.175-1522742093


APA (American Psychological Association) Style

Ullah, H., Shah, . S. Q., Khan, . A. U., Ahmad, . S. & Khattak, . M. R. (2018) In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pakistan Journal of Nuclear Medicine, 8 (1), 31-37. doi:10.24911/PJNMed.175-1522742093


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