E-ISSN 2305-1620 | ISSN 2221-0288
 
2023, Vol: 13, Issue: 1
13 / 1Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles
Required files to be uploaded

Title page (Sample)

Original Article

Online Publishing Date:
07 / 01 / 2019

 


In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications

Hameed Ullah, Syed Qaiser Shah, Aakif Ullah Khan, Shakil Ahmad, Muhammad Rauf Khattak.


Abstract
Background: A convenient method was developed for the preparation of pentavalent 99mTc dimercaptosuccinic acid using a commercially available meso-2,3-dimercaptosuccinic acid (DMSA) kit. The effects of various parameters, including incubation time, pH, and concentration of sodium bicarbonate on radiochemical purity (RCP) yield were evaluated. To determine tri- and pentavalent fractions, RCP yield of the labeled DMSA was measured using different solvent systems. Methods: The RCP yield was studied using a thin-layer chromatography on silica gel as the stationary phase and n-butanol/acetic acid/distilled water (3:2:2) as the mobile phase. Results: It was observed that hydrolyzed 99mTcO2 and 99mTc(III)-DMSA remained at the origin (Rf = 0–0.1), and 99mTc(V)-DMSA at (Rf = 0.5–0.6), while free pertechnetate, 99mTcO−4 moved to the solvent front (Rf = 0.9–1). However, in acetone, 99mTc(III)-DMSA, 99mTc(V)DMSA, and hydrolyzed 99mTcO2 stayed at the origin (Rf = 0–0.1), while free pertechnetate, 99mTcO−4 moved to the solvent front (Rf = 0.9–1). When developed with 5% glycine, 99mTcO2 stayed at the origin and the other species migrated to the solvent front. The labeling efficiency of the 99mTc(V)DMSA was 96.6± 0.5% and was stable for 24 hours at room temperature. Conclusion: Higher labeling yield, easy preparation with low cost, and good stability make 99mTc(V)-DMSA, a promising radio drug for clinical use.

Key words: Medullary thyroid cancer, 99mTc(V)-DMSA, radiochemical purity, thin-layer chromatography.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Hameed Ullah
Articles by Syed Qaiser Shah
Articles by Aakif Ullah Khan
Articles by Shakil Ahmad
Articles by Muhammad Rauf Khattak
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Ullah H, Shah SQ, Khan AU, Ahmad S, Khattak MR. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pak J Nucl Med. 2018; 8(1): 31-37. doi:10.24911/PJNMed.175-1522742093

Web Style

Ullah H, Shah SQ, Khan AU, Ahmad S, Khattak MR. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. https://www.pjnmed.com/?mno=295574 [Access: February 27, 2024]. doi:10.24911/PJNMed.175-1522742093

AMA (American Medical Association) Style

Ullah H, Shah SQ, Khan AU, Ahmad S, Khattak MR. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pak J Nucl Med. 2018; 8(1): 31-37. doi:10.24911/PJNMed.175-1522742093


Vancouver/ICMJE Style

Ullah H, Shah SQ, Khan AU, Ahmad S, Khattak MR. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pak J Nucl Med. (2018), [cited February 27, 2024]; 8(1): 31-37. doi:10.24911/PJNMed.175-1522742093


Harvard Style

Ullah, H., Shah, . S. Q., Khan, . A. U., Ahmad, . S. & Khattak, . M. R. (2018) In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pak J Nucl Med, 8 (1), 31-37. doi:10.24911/PJNMed.175-1522742093


Turabian Style

Ullah, Hameed, Syed Qaiser Shah, Aakif Ullah Khan, Shakil Ahmad, and Muhammad Rauf Khattak. 2018. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pakistan Journal of Nuclear Medicine, 8 (1), 31-37. doi:10.24911/PJNMed.175-1522742093


Chicago Style

Ullah, Hameed, Syed Qaiser Shah, Aakif Ullah Khan, Shakil Ahmad, and Muhammad Rauf Khattak. "In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications." Pakistan Journal of Nuclear Medicine 8 (2018), 31-37. doi:10.24911/PJNMed.175-1522742093


MLA (The Modern Language Association) Style

Ullah, Hameed, Syed Qaiser Shah, Aakif Ullah Khan, Shakil Ahmad, and Muhammad Rauf Khattak. "In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications." Pakistan Journal of Nuclear Medicine 8.1 (2018), 31-37. Print. doi:10.24911/PJNMed.175-1522742093


APA (American Psychological Association) Style

Ullah, H., Shah, . S. Q., Khan, . A. U., Ahmad, . S. & Khattak, . M. R. (2018) In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pakistan Journal of Nuclear Medicine, 8 (1), 31-37. doi:10.24911/PJNMed.175-1522742093




Most Viewed Articles
Most Accessed Articles

  • Determination of the optimum filter for 99mTc SPECT breast imaging using a wire mesh collimator
    Xianling Dong, M I Saripan, R Mahmud, S Mashohor, Aihui Wang
    Pak J Nucl Med. 2017; 7(1): 9-15
    » Abstract » doi: 10.24911/PJNMed.7.2

  • Behaviour of wedges for different field sizes and depths
    Sajjad Ahmed Memon, Naeem Ahmed Laghari, Fayaz Hussain Mangi
    Pak J Nucl Med. 2017; 7(1): 20-27
    » Abstract » doi: 10.24911/PJNMed.7.4

  • Pre-operative cardiac risk stratification for noncardiac surgery in cancer patients using myocardial perfusion scintigraphy
    Iqbal Munir, Amrah Javaid, Khalid Nawaz, Mohammed Hathaf Al-Rowaily, Muaadh Abdualrehman Al-Asbahi
    Pak J Nucl Med. 2017; 7(1): 28-33
    » Abstract » doi: 10.24911/PJNMed.7.5

  • Clinical characteristics and long-term outcome of patients with differentiated carcinoma of thyroid with bone metastases: a retrospective study
    Sadaf Tufail Butt, Shazia Fatima, Noreen Marwat, Kahkashan Mir, Ayesha Ammar, Mohammad Faheem
    Pak J Nucl Med. 2017; 7(1): 34-37
    » Abstract » doi: 10.24911/PJNMed.7.6

  • A brief review of targetted radionuclide therapies
    Mohammed Hathaf Al-Rowaily, Daniele Dondi, MarcoChinol, Iqbal Munir, Muaadh Abdualrehman Alasbahi
    Pak J Nucl Med. 2017; 7(1): 4-8
    » Abstract » doi: 10.24911/PJNMed.7.1

    •
    Most Downloaded
    Top Downloaded Articles

  • Behaviour of wedges for different field sizes and depths
    Sajjad Ahmed Memon, Naeem Ahmed Laghari, Fayaz Hussain Mangi
    Pak J Nucl Med. 2017; 7(1): 20-27
    » Abstract » doi: 10.24911/PJNMed.7.4

  • Determination of the optimum filter for 99mTc SPECT breast imaging using a wire mesh collimator
    Xianling Dong, M I Saripan, R Mahmud, S Mashohor, Aihui Wang
    Pak J Nucl Med. 2017; 7(1): 9-15
    » Abstract » doi: 10.24911/PJNMed.7.2

  • Characterization of SIEMENS Symbia T SPECT camera in Monte Carlo simulation environment
    Xianling Dong, Mahmud Iqbal Saripan, Rozi Mahmud, Syamsiah Mashohor, Aihui Wang,
    Pak J Nucl Med. 2018; 8(1): 18-26
    » Abstract » doi: 10.24911/PJNMed.175-1540569779

  • A historic review of the discovery of the medical uses of radioiodine
    Barbara Hertz, Pushan Bharadwaj, Bennett Greenspan
    Pak J Nucl Med. 2020; 10(1): 60-63
    » Abstract » doi: 10.24911/PJNMed.175-1582813482

  • A brief review of targetted radionuclide therapies
    Mohammed Hathaf Al-Rowaily, Daniele Dondi, MarcoChinol, Iqbal Munir, Muaadh Abdualrehman Alasbahi
    Pak J Nucl Med. 2017; 7(1): 4-8
    » Abstract » doi: 10.24911/PJNMed.7.1

    •
    Most Cited Articles
    Most Cited Articles

    About Pakistan Journal of Nuclear Medicine

    The Pakistan Journal of Nuclear Medicine (PJNM) is the official journal of the Pakistan Society of Nuclear Medicine and the first nuclear medicine journal ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Address: Pakistan Journal of Nuclear Medicine (PJNM), Room # D-115, D-Block, Department of Medical Sciences, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, 45650, Pakistan.

    Email: pjnm@pieas.edu.pk, Phone: +92 51 9248706, Fax: +92 51 9248600

    Title Owner: Pakistan Society of Nuclear Medicine

    https://psnmed.com/

    Society Email: secretary@psnmed.com

    Address: Institute of Nuclear Medicine & Oncology (INMOL), New Campus Road, Lahore, Pakistan.

    Contact the publisher:

    contact@discoverpublish.com

    https://discoverpublish.com/ 

    Address: Discover STM Publishing Ltd, 82 Leslie's Arch, Old Quarter, Ballincollig, Cork, P31 RY62, Ireland.

    Hours:  M-F: 8:30am - 5:30pm

    Contact Emails:

    Journal Editorial Office: contact@pjnmed.com

    Billing Support:  contact@discoverpublish.com

    Technical Support: anastasiia.pavlenco@sofiafields.com - https://sofiafields.com/ 


     
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use