E-ISSN 2305-1620 | ISSN 2221-0288
 

Original Research (Original Article) 


In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications

Hameed Ullah, Syed Qaiser Shah, Aakif Ullah Khan, Shakil Ahmad, Muhammad Rauf Khattak.

Abstract
Background: A convenient method was developed for the preparation of pentavalent 99mTc dimercaptosuccinic acid using a commercially available meso-2,3-dimercaptosuccinic acid (DMSA) kit. The effects of various parameters, including incubation time, pH, and concentration of sodium bicarbonate on radiochemical purity (RCP) yield were evaluated. To determine tri- and pentavalent fractions, RCP yield of the labeled DMSA was measured using different solvent systems. Methods: The RCP yield was studied using a thin-layer chromatography on silica gel as the stationary phase and n-butanol/acetic acid/distilled water (3:2:2) as the mobile phase. Results: It was observed that hydrolyzed 99mTcO2 and 99mTc(III)-DMSA remained at the origin (Rf = 00.1), and 99mTc(V)-DMSA at (Rf = 0.50.6), while free pertechnetate, 99mTcO−4 moved to the solvent front (Rf = 0.91). However, in acetone, 99mTc(III)-DMSA, 99mTc(V)DMSA, and hydrolyzed 99mTcO2 stayed at the origin (Rf = 00.1), while free pertechnetate, 99mTcO−4 moved to the solvent front (Rf = 0.91). When developed with 5% glycine, 99mTcO2 stayed at the origin and the other species migrated to the solvent front. The labeling efficiency of the 99mTc(V)DMSA was 96.6 0.5% and was stable for 24 hours at room temperature. Conclusion: Higher labeling yield, easy preparation with low cost, and good stability make 99mTc(V)-DMSA, a promising radio drug for clinical use.

Key words: Medullary thyroid cancer, 99mTc(V)-DMSA, radiochemical purity, thin-layer chromatography.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Hameed Ullah
Articles by Syed Qaiser Shah
Articles by Aakif Ullah Khan
Articles by Shakil Ahmad
Articles by Muhammad Rauf Khattak
on Google
on Google Scholar
Article Statistics
 Viewed: 1969
Downloaded: 190


How to Cite this Article
Pubmed Style

Ullah H, Shah SQ, Khan AU, Ahmad S, Khattak MR. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pak J Nucl Med. 2018; 8(1): 31-37. doi:10.24911/PJNMed.175-1522742093


Web Style

Ullah H, Shah SQ, Khan AU, Ahmad S, Khattak MR. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. http://www.pjnmed.com/?mno=295574 [Access: January 21, 2020]. doi:10.24911/PJNMed.175-1522742093


AMA (American Medical Association) Style

Ullah H, Shah SQ, Khan AU, Ahmad S, Khattak MR. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pak J Nucl Med. 2018; 8(1): 31-37. doi:10.24911/PJNMed.175-1522742093



Vancouver/ICMJE Style

Ullah H, Shah SQ, Khan AU, Ahmad S, Khattak MR. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pak J Nucl Med. (2018), [cited January 21, 2020]; 8(1): 31-37. doi:10.24911/PJNMed.175-1522742093



Harvard Style

Ullah, H., Shah, . S. Q., Khan, . A. U., Ahmad, . S. & Khattak, . M. R. (2018) In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pak J Nucl Med, 8 (1), 31-37. doi:10.24911/PJNMed.175-1522742093



Turabian Style

Ullah, Hameed, Syed Qaiser Shah, Aakif Ullah Khan, Shakil Ahmad, and Muhammad Rauf Khattak. 2018. In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pakistan Journal of Nuclear Medicine, 8 (1), 31-37. doi:10.24911/PJNMed.175-1522742093



Chicago Style

Ullah, Hameed, Syed Qaiser Shah, Aakif Ullah Khan, Shakil Ahmad, and Muhammad Rauf Khattak. "In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications." Pakistan Journal of Nuclear Medicine 8 (2018), 31-37. doi:10.24911/PJNMed.175-1522742093



MLA (The Modern Language Association) Style

Ullah, Hameed, Syed Qaiser Shah, Aakif Ullah Khan, Shakil Ahmad, and Muhammad Rauf Khattak. "In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications." Pakistan Journal of Nuclear Medicine 8.1 (2018), 31-37. Print. doi:10.24911/PJNMed.175-1522742093



APA (American Psychological Association) Style

Ullah, H., Shah, . S. Q., Khan, . A. U., Ahmad, . S. & Khattak, . M. R. (2018) In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications. Pakistan Journal of Nuclear Medicine, 8 (1), 31-37. doi:10.24911/PJNMed.175-1522742093





Most Viewed Articles
  • Behaviour of wedges for different field sizes and depths
    Sajjad Ahmed Memon, Naeem Ahmed Laghari, Fayaz Hussain Mangi
    Pak J Nucl Med. 2017; 7(1): 20-27
    » Abstract » doi: 10.24911/PJNMed.7.4

  • Determination of the optimum filter for 99mTc SPECT breast imaging using a wire mesh collimator
    Xianling Dong, M.I. Saripan, R. Mahmud, S. Mashohor, Aihui Wang
    Pak J Nucl Med. 2017; 7(1): 9-15
    » Abstract » doi: 10.24911/PJNMed.7.2

  • Clinical characteristics and long-term outcome of patients with differentiated carcinoma of thyroid with bone metastases: a retrospective study
    Sadaf Tufail Butt, Shazia Fatima, Noreen Marwat, Kahkashan Mir, Ayesha Ammar, Mohammad Faheem
    Pak J Nucl Med. 2017; 7(1): 34-37
    » Abstract » doi: 10.24911/PJNMed.7.6

  • Pre-operative cardiac risk stratification for noncardiac surgery in cancer patients using myocardial perfusion scintigraphy
    Iqbal Munir, Amrah Javaid, Khalid Nawaz, Mohammed Hathaf Al-Rowaily, Muaadh Abdualrehman Al-Asbahi
    Pak J Nucl Med. 2017; 7(1): 28-33
    » Abstract » doi: 10.24911/PJNMed.7.5

  • A brief review of targetted radionuclide therapies
    Mohammed Hathaf Al-Rowaily, Daniele Dondi, MarcoChinol, Iqbal Munir, Muaadh Abdualrehman Alasbahi
    Pak J Nucl Med. 2017; 7(1): 4-8
    » Abstract » doi: 10.24911/PJNMed.7.1

  • Bone scintigraphy in apatite-associated destructive arthritis (Milwaukee shoulder syndrome)
    Qaisar Hussain Siraj
    Pak J Nucl Med. 2017; 7(1): 44-47
    » Abstract » doi: 10.24911/PJNMed.7.9

  • Measurement of radiation doses to occupational workers in nuclear medicine
    Misbah Javed, Saeed Ur Rahman, Iqra Tanveer, Ghulam Asghar, Shazia Fatima, Mohammad Fahim
    Pak J Nucl Med. 2017; 7(1): 16-19
    » Abstract » doi: 10.24911/PJNMed.7.3

  • Successful Ra-223 treatment with a long interval between the second and the third cycles: a case report
    Tatiana Kochetova, Valeriy Krylov
    Pak J Nucl Med. 2017; 7(1): 38-41
    » Abstract » doi: 10.24911/PJNMed.7.7

  • Cutaneous flow pattern of primary lymphoedema
    Qaisar Hussain Siraj
    Pak J Nucl Med. 2017; 7(1): 42-43
    » Abstract » doi: 10.24911/PJNMed.7.8

  • Unusual breast radioiodine uptake in a nonbreastfeeding woman with papillary thyroid carcinoma
    Yassir Benameur, Hasnae Guerrouj, Salah Nabih Oueriagli,Omar Ait Sahel, Imad Ghfir, Nouzha Ben Ras Aouad
    Pak J Nucl Med. 2017; 7(1): 51-52
    » Abstract » doi: 10.24911/PJNMed.7.11

  • Most Downloaded
  • Determination of the optimum filter for 99mTc SPECT breast imaging using a wire mesh collimator
    Xianling Dong, M.I. Saripan, R. Mahmud, S. Mashohor, Aihui Wang
    Pak J Nucl Med. 2017; 7(1): 9-15
    » Abstract » doi: 10.24911/PJNMed.7.2

  • Clinical characteristics and long-term outcome of patients with differentiated carcinoma of thyroid with bone metastases: a retrospective study
    Sadaf Tufail Butt, Shazia Fatima, Noreen Marwat, Kahkashan Mir, Ayesha Ammar, Mohammad Faheem
    Pak J Nucl Med. 2017; 7(1): 34-37
    » Abstract » doi: 10.24911/PJNMed.7.6

  • A brief review of targetted radionuclide therapies
    Mohammed Hathaf Al-Rowaily, Daniele Dondi, MarcoChinol, Iqbal Munir, Muaadh Abdualrehman Alasbahi
    Pak J Nucl Med. 2017; 7(1): 4-8
    » Abstract » doi: 10.24911/PJNMed.7.1

  • Successful Ra-223 treatment with a long interval between the second and the third cycles: a case report
    Tatiana Kochetova, Valeriy Krylov
    Pak J Nucl Med. 2017; 7(1): 38-41
    » Abstract » doi: 10.24911/PJNMed.7.7

  • Measurement of radiation doses to occupational workers in nuclear medicine
    Misbah Javed, Saeed Ur Rahman, Iqra Tanveer, Ghulam Asghar, Shazia Fatima, Mohammad Fahim
    Pak J Nucl Med. 2017; 7(1): 16-19
    » Abstract » doi: 10.24911/PJNMed.7.3

  • Behaviour of wedges for different field sizes and depths
    Sajjad Ahmed Memon, Naeem Ahmed Laghari, Fayaz Hussain Mangi
    Pak J Nucl Med. 2017; 7(1): 20-27
    » Abstract » doi: 10.24911/PJNMed.7.4

  • Cutaneous flow pattern of primary lymphoedema
    Qaisar Hussain Siraj
    Pak J Nucl Med. 2017; 7(1): 42-43
    » Abstract » doi: 10.24911/PJNMed.7.8

  • Pre-operative cardiac risk stratification for noncardiac surgery in cancer patients using myocardial perfusion scintigraphy
    Iqbal Munir, Amrah Javaid, Khalid Nawaz, Mohammed Hathaf Al-Rowaily, Muaadh Abdualrehman Al-Asbahi
    Pak J Nucl Med. 2017; 7(1): 28-33
    » Abstract » doi: 10.24911/PJNMed.7.5

  • Unusual breast radioiodine uptake in a nonbreastfeeding woman with papillary thyroid carcinoma
    Yassir Benameur, Hasnae Guerrouj, Salah Nabih Oueriagli,Omar Ait Sahel, Imad Ghfir, Nouzha Ben Ras Aouad
    Pak J Nucl Med. 2017; 7(1): 51-52
    » Abstract » doi: 10.24911/PJNMed.7.11

  • A striking case of florid multifacial soft-tissue infection on an FDG-PET scan
    Qaisar Hussain Siraj, Amir Javaid, Nigora Rasulova
    Pak J Nucl Med. 2017; 7(1): 48-50
    » Abstract » doi: 10.24911/PJNMed.7.10

  • Most Cited Articles